Reporter

Part:BBa_K1639009:Design

Designed by: Nurgeldi Bazarov   Group: iGEM15_ATOMS-Turkiye   (2015-09-15)


LacI regulated DsRed with miR 26a and miR 375 binding sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 835
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 593
    Illegal BamHI site found at 876
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

miRNA binding sites make it possible to supress its expression by increasing miR levels. But in gastric cancer cells the levels of miR26a and miR 375 are low unlikely of normal epithelial cells. As a result expression of this protein supressed in normal cells but not in cancerous cells. To expand our part's usage area we have inserted BspEI cut sites on both sides of miR binding regions. Other teams can just remove that portion and change it with another sequence.

Source

This part is combination of lac operators derived from E.coli and DsRed protein originally found in Discosoma sp. and also this part contains miR binding sites at 3' end.

References

Xie Z., Wroblewska L., Prochazka L., Weiss R. ve Benenson Y. 2011. "Multi-Input RNAi-Based Logic Circuit for Identification of Specific Cancer Cells". Science, 333(6047), 1307-1311.